Genomic Resources

ESTs - Xenopus ESTs are massive in number and growing!

EST Clusters/BAC Library - EST clusters which can be used to predict/choose putative full length clones.

NIBB XDB - a library of in situ hybridizations of X. laevis/tropicalis genes.

Axeldb - a library of in situ hybridizations of X. laevis/tropicalis genes.

Comparative Genomics at LLNL - These tools identify regions of the genome that are evolutionarily conserved.

Horizon Discovery - Fully-sequenced Xenopus Gene Collection (XGC) and IMAGE cDNA clones contain full coding sequences of expressed genes from Xenopus laevis and Xenopus tropicalis.

Molecular Biology Research Group

Geneservice - Full length cDNA Clones can be obtained through Geneservice. These sequences have also been deposited into the NCBI database and are available by BLAST. These libraries have been cloned into CS107 into the EcoRI (5') and Not I (3') sites.

Metazome - The Center for Integrative Genomics and the JGI have created a website that clusters genes from various species as well as provides syntenic information.

Genome Sequencing - The X. tropicalis genome is being sequenced by the JGI with funding from the Department of Energy (DOE). The progress of sequencing and assembly is evaluated regularly by the Steering Committee, which makes recommendations for further improvements. Summaries of the meetings are available.

Unique Full length Expression Library - A significant effort is underway to create a complete genome-wide full length cDNA library that is in appropriate vectors for expression cloning. Given the impressive success of EST sequencing and bioinformatics (Wellcome and NIH/NCI) to cluster and identify putative full length clones, a complete Xenopus gene set appears to be an acheivable and highly-desirable goal.